A total of 2536 adult mosquitoes were collected, belonging to 9 genera and 10 species; the dominant species in the study were: Aedes quadrivittatus, Wyeomyia adelpha, Wy. arthrostigma, and Culex restuans. Highest richness was recorded in the dry season, whereas higher abundance was detected during the rainy season. The urban forest had the highest species richness (n = 7) when compared to all other sites. Species composition cluster analyses show that there is a high degree of similarity in species numbers across sites and seasons throughout the year. However, when considering the abundance of such species, the well-preserved montane cloud forest showed significantly higher abundance. Moreover, the urban forest is only 30 % similar to other sites in terms of species abundances, indicating a possible isolating role of the urban environment. Mosquito assemblage was differentially influenced by land-use change and seasonality, but at the same time the assemblage is rather homogeneous across the studied landscape, suggesting a high degree of spatial connectivity. Information generated in this study is potentially useful in the development of urban planning and surveillance programs focused mainly on mosquito species of medical and veterinary importance.DOI 10.1186/s13071-015-1086-9